Research has shown that traditional student assessment methods, ranging from subjective essays to standardized tests, face notable limitations as they can induce stress and may be susceptible to plagiarism, particularly with the rise of generative AI tools. Thus, there is a growing need for real-time measures of learning that not only reduce student stress but also efficiently detect genuine cognitive engagement as markers of learning. By leveraging the recent advances in bio-sensing, signal processing, and machine learning, we aim to uncover physiological signatures that may correlate with learning. This fusion of sensor modalities represents a departure from reliance on verbal or written student outputs, offering a potential window into learning processes in real time. In this pilot study (n=6), eye-tracking features often showed larger effect-size correlations with learning gains than facial-expression or ECG features; however, no single biomarker remained significant after correcting for multiple comparisons. The most robust result was a cross-modal interaction in which focused medium-duration, low-dispersion fixations interacted with heart-rate variability (RMSSD) to predict learning gain (FDR q < 0.05). These results are exploratory and motivate larger studies to validate single-feature and cross-modal biomarkers.This exploratory work examines assumptions about education assessments, shifting the focus from assessing learning using traditional methods that measure the current state of knowledge through oral or written submissions to capturing the change in knowledge (delta) in the student during the course intervention. These preliminary findings suggest that real-time physiological biomarkers may open up new avenues for personalized education, though further research with larger samples is needed to validate these initial observations.
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